invivoid - 3D Cell Culture Technology

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invivoid® - An innovative tissue engineering technology that will achieve breakthrough in the next-generation drug discovery

"invivoid®", an innovative 3D cell culture technology, that TOPPAN Holdings Inc. has developed in collaboration with Prof. Matsusaki of Osaka University, enables co-culturing of various types of cells using our proprietary cell culture methods, while controlling the behavior of cells.
Among a wide range of potential applications including drug discovery and regenerative medicine, invivoid® enables groundbreaking in vitro 3D cancer / stromal models that achieve both reproduction of tumor microenvironment and high testing throughput.

Applications in Oncology Research
Applications in
Oncology Research
 Applications in Personalized Medicine
Application in
Personalized Medicine
 Applications in Toxicology Research
Applications in
Toxicology Research
 Applications in Regenerative Medicine
Applications in
Regenerative Medicine
 Applications in Cultivated Meat
Applications in
Cultivated Meat
 invivoid® Technology
Technology
invivoid® Technology

Research Collaborations

  • Osaka University
    • Prof. Michiya Matsusaki, Division of Applied Chemistry, Graduate School of Engineering
  • Japanese Foundation for Cancer Research
    • Dr. Naoya Fujita, Center Director, Cancer Chemotherapy Center
    • Dr. Ryohei Katayama, Chief, Division of Experimental Chemotherapy, Cancer Chemotherapy Center
    • Dr. Kensei Yamaguchi, Deputy Hospital Director, Department Director of Gastroenterological Chemotherapy, Cancer Institute Hospital
    • Dr. Eiji Shinozaki, Vice Director, Gastroenterological Chemotherapy Department, Cancer Institute Hospital

NEWS

INFORMATION
The "Consortium for Future Innovation by Cultured Meat" will exhibit actual cultured meat as "homemade marbled meat" and a meat maker at the Osaka Healthcare Pavilion at the Osaka-Kansai Expo
NEWS RELEASE
Osaka University, TOPPAN Holdings, the Food Science Institute, and Meiji Achieve in Vitro Creation of Mammary Gland Tissue Capable of Producing Milk-like Substances
NEWS RELEASE
TOPPAN Holdings Announces Collaborative Research Agreement to Evaluate Potential Applications of Cell Culture Technology
TOPICS
R&D on Social Implementation of Cultured Wagyu Beef Selected for Promotion Under NEDO’s Project for Development of Innovative Biomanufacturing Technologies
TOPICS
JFCR, Osaka University, and Toppan Begin Clinical Research for Optimal Selection of Anticancer Drugs
TOPICS
Osaka University, Shimadzu, Itoham Yonekyu, Toppan, and SIGMAXYZ have established “the future creation consortium for cultured meat.”
TOPICS
Toppan and Myoridge to Collaborate on Products, Services, and R&D in the Field of Cell Culture
TOPICS
Research Paper on Original Tissue Modeling Technology Using 3D Printing Named in Nature Communications 2021 Top 25 Life and Biological Sciences Articles
NEWS RELEASE
Research Paper on Original Tissue Modeling Technology Using 3D Printing Featured in Nature Communications
TOPICS
Promising Advances in Breast Regeneration Therapy

Publications

  • Posters
    • Development of anisotropic fibers by simultaneous spinning of cells and collagen using phase separationI(ISCCM2024)
    • Construction of a 3D mammary gland model using collagen microfiber(WBC2024)
    • Development of 3D stromal tissue model for in vitro evaluating the behavior of immune cells(The 83rd JCA, 2024)
    • Development of a 3D layered co-culture model using primary cultures for drug sensitivity testing in personalized medicine(13th AACR-JCA Joint Conference, 2024)
    • Development of A Unique Drug Sensitivity Test Using Primary Culture CRC Clinical Specimen and 3D Co-culture Model(The 8th JCA-AACR Special Joint Conference, 2024)
    • Development of 3D stromal tissue model for in vitro evaluating the behavior of immune cells(The 1st International Symposium on Cancer Immunology and Immunotherapy, 2023)
    • Drug screening by layered 3D co-cultured tumor model including vascularized stromal tissue(AACR2023)
    • Construction of 3D mammary gland tissues that can be co-cultured with mature adipocytes(FASEB2023)
    • Cancer cell character alteration cultured in a novel collagen microfiber with high dispersibility could be retained after the removal of scaffold(SFB2023)
    • Layered 3D co-cultured tumor model including vascularized stromal tissue may reflect drug sensitivities in vivo tumor(AACR2022)
    • Construction of Hepatic Vascular Model and Toxicity Assessment System That Can Predict DILI Compounds(Pacifichem2021)
    • Construction of Hepatic Vascular Model and Toxicity Assessment System That Can Predict DILI Compounds(SOT2021)
    • Unique layered 3D stromal tissue model for in vitro evaluating the behavior of immune cells in cancer microenvironment(JCA2021)
    • Construction of Hepatic Vascular Model and Toxicity Assessment System That Can Predict DILI Compounds(SOT2020)
    • A unique ex vivo tumor model: 3D co-cultured system with cancer and stromal cells including blood microvessels(ASCO-GI2020)
    • A unique layered 3D stromal tissue model for in vitro evaluation of immunologic agents and immune cell infiltration into microenvironment(AACR2020)
    • A unique ex vivo tumor model: 3D co-cultured system with cancer cells and stromal tissue for drug evaluation(AACR2020)
    • Development of an in vitro Vascularized Gingival Tissue Models for P. gingivalis Infection(BEMS2019)
    • A unique layered 3D stromal tissue model for in vitro evaluation of immunologic agents and immune cell infiltration into cancer microenvironment(AACR 2018)
    • A unique ex vivo drug evaluation model: 3D co-cultured system with tumor, stroma and blood micro-vessels(AACR2018)
  • Peer-Reviewed Papers
    • Maruyama K. et al., NPJ Precis Oncol. 2025 Jan 6;9(1):4. doi: 10.1038/s41698-024-00793-6.
    • Sato K. et al., biomedicines 2024 Oct 23 (22):2500 10.3390/biomedicines12112500
    • Piantno, M et al,, Trends Biotechnol., 2024 Sep 43 (2) 364-382 doi: 10.1016/j.tibtech.2024.08.008
    • Sasaki N. et al., Acta Biomater. 2024 Sep 1;185:161-172. doi:10.1016/j.actbio.2024.07.001.
    • Shiraishi, A. et al., Biochem Biophys Res Commun. 2024 Sep 17:725:150255. doi: 10.1016/j.bbrc.2024.150255.
    • Takahashi, Y., Morimura, R. et al., Acta Biomater. 2024 Jul 15:183:111-129. doi: 10.1016/j.actbio.2024.05.037.
    • Yamada A., et al., Mater Today Bio. 2024 Jun 26 :101097 10.1016/j.mtbio.2024.101097
    • Louis F et al., Mater Today Bio. 2023 Aug 21:100720 doi: 10.1016/j.mtbio.2023.100720
    • Suezawa T. et al., Advanced Science, 2023 Mar 23 doi.org/10.1101/2023.03.21.533590
    • Shang Y. et al., Bull. Chem. Soc. Jpn. 2022, 95, 1163–1168 | doi:10.1246/bcsj.20220108
    • Naito Y et al., Acta Biomater. 2022 Mar 1;140:275-288. doi:10.1016/j.actbio.2021.11.027.
    • Louis F et al., Adv Healthc Mater. 2022 Dec;11(23):e2201440. doi: 10.1002/adhm.202201440.
    • Sasaki N. et al., Biomater Sci. 2021 Sep 28;9(19):6574-6583. doi: 10.1039/d1bm00831e.
    • Louis F et al., Bioact Mater. 2021 May 30;7:227-241. doi: 10.1016/j.bioactmat.2021.05.020.
    • Kang DH et al., Nat Commun. 2021 Aug 24;12(1):5059. doi: 10.1038/s41467-021-25236-9.
    • Hassan G et al., Processes 2021, 9(1), 45; https://doi.org/10.3390/pr9010045
    • Louis F et al., Cyborg Bionic Syst. 2021 Mar 13;2021:1412542. doi:10.34133/2021/1412542.
    • Nakatsuji H et al., Mater Horiz. 2021 Apr 1;8(4):1216-1221. doi: 10.1039/d0mh02058c.
    • Louis F et al., Biochem Biophys Res Commun. 2020 Feb 17:S0006-291X(20)30286-2. doi:10.1016/j.bbrc.2020.02.020.
    • Yanagisawa K et al., Biology (Basel). 2020 Oct 27;9(11):361. doi:10.3390/biology9110361.
    • Liu H et al., Adv Biosyst. 2020 May;4(5):e2000038. doi:10.1002/adbi.202000038.
    • Nakatsuji H et al., Curr Protoc Cell Biol. 2020 Sep;88(1):e112. doi: 10.1002/cpcb.112.
    • Li J et al., ACS Biomater Sci Eng. 2020 Oct 12;6(10):5711-5719. doi:10.1021/acsbiomaterials.0c00427.
    • Naka Y et al., Mater Today Bio. 2020 Apr 28;6:100054. doi: 10.1016/j.mtbio.2020.100054.
    • Louis F et al., Acta Biomater. 2019 Jan 15;84:194-207. doi:10.1016/j.actbio.2018.11.048.
    • Takeuchi H et al., PLoS Pathog. 2019 Nov 7;15(11):e1008124. doi: 10.1371/journal.ppat.1008124.
    • Nakatsuji H and Matsusaki M, ACS Biomater Sci Eng. 2019 Nov 11;5(11):5610-5614. doi:10.1021/acsbiomaterials.9b00090.


Posters can be downloaded via CONTACT page.

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