By adding heparin and type I collagen to cell suspension, a viscous body is formed by the strong aggregation of cells after centrifugation. Adhesion of cells is induced by the collagen, resulting in three-dimensional tissue. Various types of cells in the tissue mutually interact and align themselves, creating tissue with structures such as a network of capillary blood vessels.
A layer of cells up to 300 μm thickness can be fabricated in one step. Tissues with multi-layer structure can easily be constructed by repeating this step.

Layered Co-Culture

Cell positions in layered tissue can be designed, constructed, and maintained during culture.

Example of tissue with five-layer structure

Self-Assembled Structures

Self-assembled structures, such as capillary blood vessels with vascular endothelial cells, can be formed.

Example of co-cultured liver tissue with sinusoidal network
(green: anti-albumin antibody [Hepatocyte], red: anti-CD31 antibody [Sinusoidal Endothelial Cells])

Diverse Cell Sources

- Patient-derived cells
- Cell lines
- iPSC / ESC-derived cells
- etc.

Example of culturing patient-derived primary cancer cells. Cancer cells grew only in invivoid^® co-culture model using inexpensive growth factor free medium (DMEM).